Phase-Contrast Cryo-Electron Tomography of Primary Cultured Neuronal Cells

نویسندگان

  • Yoshiyuki Fukuda
  • Radostin Danev
  • Shoh Asano
  • Miroslava Schaffer
  • Florian Beck
  • Vladan Lučic
  • Wolfgang Baumeister
چکیده

To understand synaptic function, unveiling molecular architecture of synapse is one of the important issues. TEM observation of plastic embedded specimens stained with heavy metals has fundamentally contributed to our knowledge about basic structural features of neuronal synapses. However, structural investigations of synapse at the close-to-physiological state are required for a further understanding of synaptic architecture and function.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Zernike Phase Contrast Cryo-Electron Tomography of Bacteria and Viruses

Cryo-electron tomography (cryo-ET) is currently the only method available through which pleomorphic biological specimens are imaged to produce three-dimensional (3D) structures at ~2 to 10 nm resolution [1]. There remain challenges associated with imaging intact bacteria and pleomorphic viruses by cryo-ET. In particular, investigators cope with the low contrast of cryo-preserved, unstained spec...

متن کامل

Artifact Correction for Zernike Phase-Plate Cryo-Electron Tomography

In cryo-electron microscopy (cryo-EM), vitreously frozen biological specimens can tolerate only a limited electron dose for imaging. Therefore, cryo-EM micrographs display weak contrast and a poor signal-to-noise ratio. This limits data interpretability and prevents efficient application of cryo-EM to a wider range of biological problems. Continued efforts have been invested in imaging methods ...

متن کامل

Phase Contrast Subtomogram Averaging at Sub-nm Resolution

Phase contrast imaging is a powerful technique that can increase the signal to noise ratio and contrast of the phase objects in their native and frozen hydrated state. The improved contrast is as a result of the phase shift between the unscattered and the scattered electron beams. The so called Volta phase plate (VPP) is a newly developed thin film phase plate which has advantages compared to t...

متن کامل

Insights into the molecular organization of the neuron by cryo-electron tomography.

Despite great progress in the identification and characterization of the key molecular players in neuronal function, remarkably little is known about their supramolecular organization. Cryo-electron tomography (cryo-ET), providing three-dimensional views of the molecular components of the cell in their native, fully hydrated environment, is uniquely positioned to elucidate the native architectu...

متن کامل

Phase Contrast Cryo-Electron Tomography and Single Particle Analysis with a New Phase Plate

Biological samples are very sensitive to beam irradiation because of induced changes to their structure by the incident electron. Due to the limited allowed dose, cryo-images of frozen-hydrated biological specimens are typically noisy and low in contrast. One way to improve the imaging of such samples is by using a phase plate to increase contrast and signal-to-noise ratio. The improved contras...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2014